Fertilization and development of mouse oocytes cryopreserved using a theoretically optimized protocol
نویسندگان
چکیده
منابع مشابه
Fertilization and Embryo Development of Fresh and Cryopreserved Sibling Oocytes
Background The pattern of Islamic fasting differs from other forms of fasting, therefore its effect on health also differs. This research studies the effect of Islamic fasting on gonadotropin hormones around the time of the ovulatory cycle and ovulation. MaterialsAndMethods This self-controlled study was performed on 24 adult females. Blood sampling was performed during Ramadan and two months l...
متن کاملfertilization and embryo development of fresh and cryopreserved sibling oocytes
background: the pattern of islamic fasting differs from other forms of fasting, therefore its effect on health also differs. this research studies the effect of islamic fasting on gonadotropin hormones around the time of the ovulatory cycle and ovulation. materials and methods: this self-controlled study was performed on 24 adult females. blood sampling was performed during ramadan and two mont...
متن کاملProlonged exposure to hyaluronidase decreases the fertilization and development rates of fresh and cryopreserved mouse oocytes
Hyaluronidase is generally used to remove cumulus cells from mouse oocytes before oocyte cryopreservation, intracytoplasmic sperm injection or DNA injection. In general, use of cumulus-free mouse oocytes decreases in vitro fertilizing ability compared with cumulus-surrounded oocytes. The effect of hyaluronidase exposure on the quality of mouse oocytes is not fully understood. Here, we investiga...
متن کاملHigh survival of mouse oocytes using an optimized vitrification protocol
The method of vitrification has been widely used for cryopreservation. However, the effectiveness of this method for mammalian oocytes could be improved by optimizing each step of the process. In the present study, we tested the effects of varying several key parameters to determine the most effective protocol for mouse oocyte vitrification. We found that cryoprotectant containing ethylene glyc...
متن کاملMaturation, fertilization, and the structure and function of the endoplasmic reticulum in cryopreserved mouse oocytes.
Oocyte cryopreservation is a promising technology that could benefit women undergoing assisted reproduction. Most studies examining the effects of cryopreservation on fertilization and developmental competence have been done using metaphase II-stage oocytes, while fewer studies have focused on freezing oocytes at the germinal vesicle (GV) stage, followed by in vitro maturation. Herein, we exami...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 1996
ISSN: 0268-1161,1460-2350
DOI: 10.1093/oxfordjournals.humrep.a019375